Innovative Methodology Insulin uptake across the luminal membrane of the rat proximal tubule in vivo and in vitro
نویسندگان
چکیده
Pavel Kolman,* Angelo Pica,* Nicolas Carvou,* Alan Boyde, Shamshad Cockcroft, Andrew Loesch, Arnold Pizzey, Mariadelina Simeoni, Giovambattista Capasso, and Robert J. Unwin Institute of Physical Engineering, Brno University of Technology, Brno, Czech Republic; Department of Internal Medicine, Second University of Naples, Naples, Italy; and Barts and The London School of Medicine and Dentistry, Centre for Nephrology, Department of Physiology, Department of Anatomy and Developmental Biology, and Department of Haematology, University College London, London, United Kingdom
منابع مشابه
Insulin uptake across the luminal membrane of the rat proximal tubule in vivo and in vitro
We visualized insulin uptake in vivo across the apical membrane of the rat proximal tubule (PT) by confocal microscopy; we compared it with in vitro findings in a rat PT cell line (WKPT) using fluorescence microscopy and flow cytometry. Surface tubules were observed in vivo with a 633-nm single laser-illuminated real-time video-rate confocal scanning microscope in upright configuration for opti...
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